Study of Genes Expression Involved in Apoptosis (Bax, Survivin) After Induced Cold Injury on Sensorimotor Cortex of Mouse Brain and Treatment With Magnesium Chloride

Purpose: The purpose of this study was to investigate the expression of Bax and surviving after cold injury and the ability of magnesium chloride to inhibit of apoptosis. Materials and Methods: To produce cold injury, a metal probe cooled with liquid nitrogen was applied to the surface of the mouse intact skull above the parietal lobe by force of 100 gr for 30 sec. Brains were removed 72 h after cold injury and sections were prepared to study immunohistochemistry reactions of Bax and Survivin antibodies. The data were analyzed using t- tests. Results: The results indicated that expression of Bax was higher in model group comparing with treated ones. Survivin was expressed more in the treated group and there was significant difference with model group (p<0.05). Conclusion: MgCL2 with optimum dose causes activate expression of Survivin and inhibition of apoptosis.